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Gram staining is commonly used on prokaryotic bacteria to determine if their cell wall is gram negative or gram positive. Their cell wall is made of peptidoglycan. It responds well and is stained in certain ways depending on the structure (wether it's gram positive or negative).
Animal eukaryotic cells don't even have a cell wall to be stained. In the case of eukaryotic plant cells, that do have cell walls, their cell wall is not made of peptidoglycan. In any case, there is no such thing as gram positive, or gram negative, cell walls in eukaryotes. There would be no point in the test, since it's specifically designed for prokaryotic cells.
Wiki User
∙ 15y ago
Wiki User
∙ 11y agoHuman cells cannot be successfully Gram Stained, because they have no cell wall. The purple colouring of the stain just washes out of human cells.
Normally, the iodine reacts with the peptidoglycan in the bacteria's cell wall. This means that the crystal violet is not washed out. Human cells only have a plasma membrane, so the colour washes out. This means that the Grain stain would have a negative result, even if the nuclei retains some colouring.
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Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
What is gram staining?
Gram Staining is a way to separate one large groups of bacteria into two. Crystal violet is used to dye the cells, those that retain the color are grouped as Gram-positive, and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic, making this process useful for detecting infections. A link is provided to permit a quick trip to the Wikipedia article on this topic.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Are there endospores in pseudomonas aeruginosa gram staining?
yes
What are four possible results of a gram stain?
The four possible results after Gram staining are Gram positive, Gram negative, Gram variable and Gram in-determinant.
Why is Gram of gram stain written in capital letters?
Proper nouns are usually capitalized; the word Gram is an eponym of Hans Christian Gram, the inventor of Gram staining, hence capitalized.
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
